oligo binding buffer (Zymo Research)
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oligo binding buffer
Oligo Binding Buffer, supplied by Zymo Research, used in various techniques. Bioz Stars score: 95/100, based on 58 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/oligo+binding+buffer/Oligo+Binding+Buffer/us12601006-265-27-30
Average 95 stars, based on 58 article reviews
Oligo Binding Buffer, supplied by Zymo Research, used in various techniques. Bioz Stars score: 95/100, based on 58 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/oligo+binding+buffer/Oligo+Binding+Buffer/us12601006-265-27-30
Average 95 stars, based on 58 article reviews
oligo binding buffer - by Bioz Stars,
2026-09
95/100 stars
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other:Article Title: The functional organization of chromosome territories in single nuclei during zygotic genome activation. Article Snippet: The designed library was amplified using Kapa Taq enzyme (Kapa Biosystems, 5 U/μl), with the following PCR program: 95 C, 5 min, (95 C for 30 s, 58 C for 30 s, 72 C for 20 s) repeated 12 times, 72 C 5 min, hold at 4 C. The linear PCR products were purified using Binding Assay:Article Title: Deciphering chromosome fusion in D. miranda’s neo-sex chromosome through single-copy and repetitive oligo probes Article Snippet: .. 240 μl of Article Title: Targeted, long-read nucleic acid sequencing for the determination of cytosine modifications Article Snippet: .. The sample was incubated at 37° C. and 850 rpm in a ThermoMixer (Eppendorf) for 16 h. The reaction was purified on Zymo-IC column (Zymo Research) with Article Title: Genome-wide extraction of differentially methylated DNA regions using adapter-anchored proximity primers Article Snippet: Oxidized DNA was then reduced in a 50 μl of reaction containing 100 mM sodium acetate solution (pH = 4.0) and 100 mM pic-borane (Alfa Aesar) for 6 h at 37°C and 850 rpm in the Eppendorf ThermoMixer. .. The product was purified by Zymo-IC column with Article Title: The functional organization of chromosome territories in single nuclei during zygotic genome activation Article Snippet: Using the reverse transcriptase Maxima H Minus RT (ThermoFisher Scientific), RNA was reverse transcribed into DNA, and the RT enzyme was inactivated at 85 °C for 5 min. After the inactivation of the RT enzyme, all RNA in the solution was degraded using alkaline hydrolysis (0.5 M EDTA and 1 M NaOH in 1:1) at 95 °C for 10 min. .. Subsequently, the oligos were purified using the same clean-up kit with the Article Title: The Fanconi anemia pathway repairs colibactin-induced DNA interstrand cross-links Article Snippet: The plates were further incubated at 37 °C without shaking for 5 h. The contents in the 96-well plates were then recombined and spun down at 4000 × g for 20 min, and the supernatant was carefully decanted and filtered through a 0.22 μM PES filter (Corning), flash frozen in liquid nitrogen, and lyophilized to complete dryness. .. Dried samples were reconstituted in Article Title: Targeted, long-read nucleic acid sequencing for the determination of cytosine modifications Article Snippet: The reaction was incubated at 30° C. for 80 min. 1 μL of Proteinase K (NEB, 0.8 μL) was added to the oxidation reaction and incubated for 1 h at 50° C. 6 μL of 3 M sodium acetate buffer solution (pH 4.3) and 3 μL of pyridine borane (Alfa Aesar, —10 M) were added to the 21 μL DNA sample (final 30 μL reaction contains 600 mM NaAc and 1 M pyridine borane). .. The solution was incubated at 37° C. and 850 rpm in a ThermoMixer (Eppendorf) for 16 h. The reaction was purified on Zymo-IC column (Zymo Research) with Incubation:Article Title: Targeted, long-read nucleic acid sequencing for the determination of cytosine modifications Article Snippet: .. The sample was incubated at 37° C. and 850 rpm in a ThermoMixer (Eppendorf) for 16 h. The reaction was purified on Zymo-IC column (Zymo Research) with Article Title: Targeted, long-read nucleic acid sequencing for the determination of cytosine modifications Article Snippet: The reaction was incubated at 30° C. for 80 min. 1 μL of Proteinase K (NEB, 0.8 μL) was added to the oxidation reaction and incubated for 1 h at 50° C. 6 μL of 3 M sodium acetate buffer solution (pH 4.3) and 3 μL of pyridine borane (Alfa Aesar, —10 M) were added to the 21 μL DNA sample (final 30 μL reaction contains 600 mM NaAc and 1 M pyridine borane). .. The solution was incubated at 37° C. and 850 rpm in a ThermoMixer (Eppendorf) for 16 h. The reaction was purified on Zymo-IC column (Zymo Research) with Purification:Article Title: Targeted, long-read nucleic acid sequencing for the determination of cytosine modifications Article Snippet: .. The sample was incubated at 37° C. and 850 rpm in a ThermoMixer (Eppendorf) for 16 h. The reaction was purified on Zymo-IC column (Zymo Research) with Article Title: Genome-wide extraction of differentially methylated DNA regions using adapter-anchored proximity primers Article Snippet: Oxidized DNA was then reduced in a 50 μl of reaction containing 100 mM sodium acetate solution (pH = 4.0) and 100 mM pic-borane (Alfa Aesar) for 6 h at 37°C and 850 rpm in the Eppendorf ThermoMixer. .. The product was purified by Zymo-IC column with Article Title: The functional organization of chromosome territories in single nuclei during zygotic genome activation Article Snippet: Using the reverse transcriptase Maxima H Minus RT (ThermoFisher Scientific), RNA was reverse transcribed into DNA, and the RT enzyme was inactivated at 85 °C for 5 min. After the inactivation of the RT enzyme, all RNA in the solution was degraded using alkaline hydrolysis (0.5 M EDTA and 1 M NaOH in 1:1) at 95 °C for 10 min. .. Subsequently, the oligos were purified using the same clean-up kit with the Article Title: The Fanconi anemia pathway repairs colibactin-induced DNA interstrand cross-links Article Snippet: The plates were further incubated at 37 °C without shaking for 5 h. The contents in the 96-well plates were then recombined and spun down at 4000 × g for 20 min, and the supernatant was carefully decanted and filtered through a 0.22 μM PES filter (Corning), flash frozen in liquid nitrogen, and lyophilized to complete dryness. .. Dried samples were reconstituted in Article Title: Targeted, long-read nucleic acid sequencing for the determination of cytosine modifications Article Snippet: The reaction was incubated at 30° C. for 80 min. 1 μL of Proteinase K (NEB, 0.8 μL) was added to the oxidation reaction and incubated for 1 h at 50° C. 6 μL of 3 M sodium acetate buffer solution (pH 4.3) and 3 μL of pyridine borane (Alfa Aesar, —10 M) were added to the 21 μL DNA sample (final 30 μL reaction contains 600 mM NaAc and 1 M pyridine borane). .. The solution was incubated at 37° C. and 850 rpm in a ThermoMixer (Eppendorf) for 16 h. The reaction was purified on Zymo-IC column (Zymo Research) with |